Biological Lab Cleanroom Design and Construction Plan

1. Layout

According to the requirements of the laboratory, the biological lab cleanroom was designed with a construction area of 23㎡ as an example. There should be a P2 main laboratory (P2 laboratory is Physical Containment Level 2 Laboratory), a one-buffer room, a two-buffer room, and a scrubbing and disinfecting room.

Plan the layout according to the relevant national regulations and the special requirements of the experimental site. Consider that the biosafety laboratory should implement the requirements of the first-level barrier and the second-level barrier at the same time. The first-level barrier is mainly reflected in the layout and maintenance structure of the microbiological experiment area. The secondary barrier is mainly reflected in the air-conditioning purification system.

1. Separate the flow of people and logistics in the plane layout to prevent cross infection of people and things.

2. The experiment operator enters the P2 main laboratory through the first buffer room and the second buffer room, and the experimental objects enter and exit the P2 main laboratory through two interlocking pass boxes.

3. One of the interlocking pass boxes is located between the main laboratory and the preparation room, and the other is located between the main laboratory and the scrubbing and disinfecting room, which ensures that the cleans are not infected, and at the same time ensures that the pollutants are passed into the scrubbing and disinfecting as soon as possible The room is sterilized.

2. Enclosure Cleanroom Materials

The design requires that the cleanroom wall of the laboratory is a cleanroom panel with a thickness of 50mm, and the core material is non-combustible. Wiskind is a supplier of modular cleanroom enclosure materials. Rockwool cleanroom panel is recommended. It features high flatness, corrosion resistance, thermal insulation and fire protection. Can meet the requirements of the overall biological lab cleanroom.

The ceiling material is also a cleanroom panel. The indoor ground of the microbial area is welded with imported PVC materials without gaps, which has the characteristics of corrosion resistance, strong wear resistance, good flatness and easy cleaning.

The ground corner line is decorated with negative angle clean room aluminum material, which is beautiful and tight. Through scientific design and careful construction, the overall laboratory will form a sterile, seamless, smooth, beautiful, non-reflective, dust-free, rust-free, moisture-proof, anti-bacterial, and high-performance sterile surface and inner shell in the laboratory. Thereby solving the problems of air tightness and cleaning.

All materials of Wiskind modular cleanroom are processed and formed in the factory according to the design drawings, and shipped to the site for rapid installation. All the equipment in the room is embedded in the wall, making the wall of the whole laboratory smooth and seamless, preventing dust accumulation, and convenient for cleaning and disinfection.

3. Basic Configuration of Microbiology Laboratory

In addition to solving the problem of air purification, the clean laboratory should provide as much convenience as possible for the experimental work, so some necessary laboratory equipment should be considered in the design.

1. Interlocking pass box: equipped with two pass box in the P2 laboratory. Ensure the safety of laboratory logistics. There is an ultraviolet lamp in the interlocking transfer window, which is disinfected before the contaminated items are taken out of the laboratory. The interlocking transfer window also ensures the isolation of outdoor and indoor air, which facilitates the transfer of items by experimenters and reduces the entry and exit of experimenters The frequency of the laboratory. Both the indoor and outdoor transmission windows can be controlled.

2. Automatic door closer: According to the requirements of national regulations, an automatic door closer is installed on the clean laboratory door.

3.Interlocking cleanroom door: The main laboratory is equipped with a set of two interlocking electronic interlocks. When one of the doors is not closed, the other door will not open. This will buffer the airflow into the clean room. The interlocking door is also equipped with an emergency switch. When an accident occurs, press the emergency switch to facilitate laboratory personnel to escape from the scene as soon as possible.

4. Socket: According to laboratory use requirements. In each laboratory, according to different experimental purposes, a sufficient number of single-phase sockets and three-phase sockets should be configured. The sockets are all embedded and air-tight. The imported multi-functional dust-proof, waterproof and anti-leakage combined power supply board is imported, and it is installed with advanced technology.

5. Ultraviolet lamps: Considering the disinfection requirements of the clean room, two ultraviolet disinfection lamps should be equipped in the P2 main laboratory to facilitate the physical disinfection before and after the experiment.

6.Sample cart: In order to facilitate laboratory personnel to move samples and other experimental supplies, a small sample cart should be specially arranged in the scrubbing and disinfecting room. It is a stainless steel structure with two layers on the top and bottom, which is small and convenient to promote.

7. Biosafety cabinet (BSC-Ⅱ-A2): According to the requirements of the national regulations of the biosafety laboratory, the P2 laboratory should be equipped with a biosafety cabinet. Biological safety cabinets are the main isolation equipment used in biological laboratories to prevent the spread of harmful suspended particles. Based on the protection provided to people, the environment and the samples of the test article, the level varies. Class II A2 biological safety cabinet can provide comprehensive protection for personnel, test samples, and environment, and is applied to the operation of high-risk microorganisms, toxic substances and radioactive oligonucleotides. A2 type A2 is a front-door biological safety cabinet, which protects the staff by vertical airflow, protects the samples by multi-layer HEPA filtration, and protects the environment by HEPA filtration exhaust gas. The airflow settles from the top of the cabinet through the HEPA intake filter. After the gas reaches the working surface, about 50% of the gas is sucked into the rear grille of the cabinet, and the remaining gas meets the indoor air at the front grille, of which 30% of the air can pass through The HEPA exhaust filter is discharged, and the remaining 70% of the air is mixed with fresh air through HEPA filter to form a sterile work area, which effectively prevents cross-contamination. A gas barrier is generated where the indoor air meets the HEPA filtered air, so that the staff and the sample are isolated, protecting the safety of the experimenter, and the sample is not contaminated.

4. P2 Biosafety Laboratory Technical Indicators (secondary barrier)

The biological clean room should strictly follow the health industry standard of the People's Republic of China WS233-2002 "General Guidelines for Biosafety in Microbiology and Biomedical Laboratories" and the National Standard GB19489-2004 "General Requirements for Laboratory Biosecurity" The assembly standard is to assemble different levels of biological clean rooms for users according to the degree of biological hazards of the biological factor analysis process carried out by various professional laboratories and according to the biological safety level classification in national standards. Called level 1, level 2, level 3, and level 4.

1. Grading standards: laboratory grade 1 grade 2 grade 3 grade 4

P1 treatment objects are less harmful to humans, animals or the environment, and do not have pathogenic factors that cause disease to healthy adults, animals and plants. P2 is a moderately hazardous or potentially dangerous pathogenic factor for humans, animals, plants or the environment, and will not cause serious harm to healthy adults, animals and the environment. Effective prevention and treatment measures.

P3 is highly dangerous to humans, animals and plants or the environment, mainly through aerosols to cause serious or even fatal diseases, or pathogenic factors that are highly harmful to animals, plants and the environment. There are usually preventive treatments.

P4 is highly dangerous to humans, animals, plants or the environment. It is transmitted by aerosol or its transmission route is unknown, or unknown and dangerous pathogenic factors. There is no preventive treatment.

2. Main technical indicators of clean laboratory

Name cleanliness class number of dust particles (particles / m3) ventilation (times / h) pressure difference (Pa) temperature ℃ relative humidity% noise dB (A) illuminance lx≥0.5μm ≥5μm second class 7 ～ 8 100000 ≤3500000 ≤29300 ≥20 ≤-10 23 ～ 30 ≤65 ≤60 300

3. Expression of cleanliness

Cleanliness class 10000 (P20,000 laboratory is ISO7 level), that is, the number of dust particles greater than or equal to 0.5μm is greater than 35000 particles / m3 (35 particles / L) to less than or equal to 350,000 particles / m3 (350 particles / L) The number of dust particles greater than or equal to 5 μm is greater than 300 particles / m3 (0.3 particles / L) to less than or equal to 3000 particles / m3 (3 particles / L).

5. Purified Air Conditioning System

The microbial clean room is equipped with a purification air-conditioning system in order to establish a micro-environment with suitable temperature, humidity, fresh air, and aseptic cleanliness to facilitate the experimental operation. The traditional air-conditioning system can only control the temperature to a certain degree, and the cleanliness cannot be completely controlled, thus causing the breeding and reproduction of bacteria. Such an air conditioning system is far from meeting the hygienic requirements of a specific clean environment in a microbiology laboratory.

After being carefully designed through a series of technical measures, the purification air-conditioning system can fully control the temperature, dust, bacteria, harmful gas concentration and airflow distribution in the laboratory, ensure the fresh air volume required by indoor personnel and reasonable indoor airflow direction Maintain the appropriate gradient pressure and directional flow throughout the microbiology laboratory, fully realize the purpose of "sterile environmental protection system", and reduce all potential infection risks during the experiment. Another outstanding feature of the structural design is that most of the indoor air conditioning equipment maintenance is performed outdoors, reducing the chance of non-experimental personnel entering the laboratory, reducing pollution, and improving the utilization rate of the laboratory.

6.Power Distribution System

The design scope of the engineering power distribution system is the lighting and power system of the laboratory and the power supply and distribution system of the air-conditioning unit of the microbiology laboratory. The design is based on "Code for Design of Power Supply and Distribution System", "Code for Design of Low-Voltage Power Distribution", "Standard for Lighting Design of Civil Buildings", "Code for Electrical Design of Civil Buildings", "Technical Specification for Building of Biosafety Laboratory", "Biosafety for Laboratory "General Requirements", "General Guidelines for Biosafety of Microbiology and Biomedical Laboratories" and the tender documents provided by the experimental site are the design basis.

Laboratory power distribution: It is designed to set up a power distribution sub-box for the biological safety laboratory, and the main circuit of the power distribution will lead to a separate circuit for power distribution, so as to ensure the reliability and safety of laboratory electricity. In addition, the power distribution of other auxiliary laboratories is carried out in a mode laid separately from the microbiological safety laboratory, thereby further ensuring the reliability of electricity use in the biosafety laboratory; the lighting distribution of the main laboratory is separated from the socket power distribution.